Ageha Yoshina
A high-schooler who offers to take care of anyone's problems for the price of 10,000 yen. He is first transported to Psyren after he uses the calling card to find out more about the whereabouts of his classmate, Sakurako Amamiya. Once in Psyren, Ageha has to pair up with Amamiya and Hiryū Asaga to get back to Earth. After narrowly defeating the cyborg Alfred, Ageha and the other survivors return to Earth. Ageha, along with Asaga, begin to train under Amamiya and Matsuri to master their abilities. Ageha's initial PSI power was fueled almost entirely by emotion, an unusual occurrence, and manifested as a massively destructive blast of pure psychic energy. During his second trip to Psyren, Ageha's power manifested as a massive black sphere that nearly destroyed his mind and almost turned his body to ash.Ageha seems to have a crush on Sakurako Amamiya, as he gets furious and beats up Kabuto Kirisaki every time he flirts with her and has been seen dreaming about her.

Sakurako Amamiya
A girl in Yoshina's class who used to be bright and lively but was nicknamed "The Ice Princess" after she became cold and hard-hearted. Yoshina suspects that Psyren may be the cause for Amamiya's change. Once in Psyren, she comes down with a sickness that immobilizes her. This forces Yoshina and Asaga to take care of her. Amamiya seems to know more about Psyren then the other humans that are trapped there and this is due to her relationship with Matsuri. She seems specialise in weapons as she always takes one to Psyren when called. Her PSI abilities are excellent and well-spread over the three forms. Her Burst is over satisfactory; she is extremely proficient in Rise; her Trance is strong enough that it is able to create a scythe in her Mind Jack: Insanity Size. She also has some skill at creating illusions to deceive her opponents. Amamiya might have a crush on Ageha, which developed upon the first return to Psyren.

Matsuri Yagumo
A famous concert pianist and veteran Psyren drifter, she was the one who taught Amamiya all about Psyren. Because her card's value is already at zero, she cannot help the main characters and instead acts in a mentor role. She is a talented pianist, though she drinks a lot and drives a motorcycle. When Ageha and Asaga first met her, she was drunk and responding to a call for assistance from Amamiya. She reacts playfully in regards to her friend Ian's crush on her.

Nemesis Q
Nemesis is a PSI-program with basic intelligence and individuality sent back from the future to select among the people those worthy of using PSI to teach them about the future, discover the truth, and stop Psyren from occurring. The creator of "Q" mentioned that her body could not withstand the time-travel transfer so she sent a program to do it for him. Her creator also embedded another order in Q: to kill those who divulge info about Psyren to protect the creator of being caught.

Asaga
"Dragon" Asaga is a tough guy who says that he came to Psyren in search of a friend. He agrees to help out Amamiya for Yoshina. After their safe return to earth, he comforts Yoshina about Amamiya being taken by an unknown friend and reveals himself to be Asaga Hiryū, Ageha's former classmate from elementary school who used to be a cry-baby (who Ageha used to pick on; humorously, Asaga got his revenge by remarking how short Ageha was compared to him). He displays more proficiency with PSI than Ageha, learning to use his abilities and controlling it faster, though that may be because Ageha's Melchsee's Door is a more advanced technique