Aono Tsukune
The male protagonist of the series, Tsukune Aono is mistakenly enrolled in an academy for monsters. He soon meets Moka Akashiya, a vampire who takes an interest in him, due to the sweet taste of his blood.
Moka Akashiya
Moka Akashiya is the first person Tsukune meets when he sets foot in the yokai realm. Exceptionally sweet and kind, Moka is a vampire, and takes a liking to Tsukune, after ingesting his blood, and becomes addicted in doing so.
Kurumu Kurono
Kurumu Kurono is a succubus, and is initially jealous of Moka's popularity overshadowing her own. She tries to seduce Tsukune and the male student body, using her charming techniques (through her succubi abilities, including her busty physique), making them her slaves. This is so she can find her "mate of fate", in order to save her diminishing species, with the assumption that Moka is blocking that path.
Yukari Sendo
Yukari Sendo is one of the smartest students at Yokai Academy, and the youngest at age 11. Yukari is a witch, and always wears a typical witch costume, though considered by some students to be a violation of the school rules. Despite ranking #1 on the examinations, she was a lonely prankster before meeting Tsukune and Moka.
Mizore Shirayuki
Mizore Shirayuki is a yuki onna. Keeping in line with Japanese mythological creatures, she has the ability to control the element of ice at will, and form clones composed of said element. Frequently seen with a lollipop in her mouth, Mizore returns to school after a delayed absence and shows an immediate interest in Tsukune.